|
KMID : 0043320090320121689
|
|
Archives of Pharmacal Research
2009 Volume.32 No. 12 p.1689 ~ p.1694
|
|
|
Rapid Identification and Characterization of Antioxidants from Ligularia fischeri
|
|
Piao Xiangshu
Piao Xiang Lan
Mi Xiao-Yuan
Tian Yan-Ze
Piao Hui Shan
Zeng Zhikai
|
|
|
Abstract
|
|
|
|
The objectives of this study were to investigate the radical-scavenging activity of Ligularia fischeri on oxidative damage by the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and to rapidly identify the active components using the bioassay-linked fractionation method. The MeOH extract and fractions of CH2Cl2, BuOH, and H2O from L. fischeri showed DPPH radical-scavenging effects in a dose-dependent manner (p < 0.01). In particular, the BuOH fraction had the most effective (p < 0.05) antioxidative capacity. The active constituents from the BuOH fraction of L. fischeri were rapidly isolated by bioassay-linked HPLC method and identified as hyperoside and 2’’-acetylhyperoside with potent antioxidant effects against the DPPH radical, with IC50 values of 1.31 and 7.09 μg/mL, respectively. They have not been reported from L. fischeri yet.
|
|
|
KEYWORD
|
|
|
Ligularia fischeri, Antioxidative activity, Bioassay-linked HPLC, Hyperoside, 2’’-acetylhyperoside
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|